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. 2004 Apr 27;2004(1):24–34. doi: 10.1155/S1110724304306017

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HMSC plated on COL I and VN express osteopontin. (a) and (b) Immunohistochemical staining of osteopontin in hMSC on day 16. Cells plated on COL I in DMEM (a) or on VN in DMEM (b) exhibit intracellular staining and deposits of osteopontin in the extracellular space. Arrows indicate concentrated aggregates of osteopontin. Bar in (a) = 50 μm, in (b) = 200 μm. (c) Western blot of cells cultured for eight days in control medium on tissue culture plastic (lane 1), COL I (lane 2), or VN (lane 3), and probed for osteopontin expression.

HMSC plated on COL I and VN express osteopontin. (a) and (b) Immunohistochemical staining of osteopontin in hMSC on day 16. Cells plated on COL I in DMEM (a) or on VN in DMEM (b) exhibit intracellular staining and deposits of osteopontin in the extracellular space. Arrows indicate concentrated aggregates of osteopontin. Bar in (a) = 50 μm, in (b) = 200 μm. (c) Western blot of cells cultured for eight days in control medium on tissue culture plastic (lane 1), COL I (lane 2), or VN (lane 3), and probed for osteopontin expression.