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HMSC plated on COL I or VN express alkaline phosphatase activity. Cells were plated on tissue culture plastic (a), COL I (c), and vitronectin (d) in control media for 16 days then stained for alkaline phosphatase activity. Cultures were plated on tissue culture plastic in osteogenic supplement medium (b) as a positive control. Cells expressing alkaline phosphatase appear dark in phase contrast micrographs (10× magnification).
