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HMSC plated on COL I or VN express osteogenic genes. Indicated genes were amplified by RT-PCR from RNA isolated from hMSC plated for eight days in control medium on tissue culture plastic (lane 1), OS medium on tissue culture plastic (lane 2), control medium on COL I (lane 3), or control medium on VN (lane 4). As a positive control, RNA from eight-day cultures of fully differentiated osteoblasts was run in parallel (lane 5).
