. 2004 Apr 27;2004(1):24–34. doi: 10.1155/S1110724304306017

Table 1.

Primers used for RT-PCR.

GENE/NCBI designation	Primer sequences	Reference

GAPDH (glyceraldehyde-3-phosphate dehydrogenase)	Forward 5′-ATGGAAATCCCATCACCATCT-3′	(Doi et al [7])
GAPDH (glyceraldehyde-3-phosphate dehydrogenase)	Reverse 5′-GGTTGAGCACAGGGTACTTTATT-3′	(Doi et al [7])

ALPL (alkaline phosphatase)	Forward 5′-GACCCTTGACCCCCACAAT-3′	(Frank et al [23])
ALPL (alkaline phosphatase)	Reverse 5′-GCTCGTACTGCATGTCCCCT-3′	(Frank et al [23])

COL I (collagen type I)	Forward 5′-CAGCCGCTTCACCTACAGC-3′	(Frank et al [23])
COL I (collagen type I)	Reverse 5′-TTTTGTATTCAATCACTGTCTTGCC-3′	(Frank et al [23])

SPP1 (osteopontin)	Forward 5′-CTCAGGCCAGTTGCAGCC-3′	(Frank et al [23])
SPP1 (osteopontin)	Reverse 5′-CAAAAGCAAATCACTGCAATTCTC-3′	(Frank et al [23])

BGLAP (bone gamma-carboxyglutamate (gla) protein/osteocalcin)	Forward 5′-GAAGCCCAGCGGTGCA-3′	(Frank et al [23])
	Reverse 5′-CACTACCTCGCTGCCCTCC-3′	(Frank et al [23])