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Osteogenic differentiation of hMSC plated on indicated substrates for 16 days, as assessed by indicated measurements of mineral to matrix ratio. By comparison, fully differentiated osteoblasts cultured on tissue culture plastic for 21 days have a ratio of approximately 5.4.
| HMSC plated on substrate/medium | Mineral to matrix ratio |
|---|---|
| Tissue culture plastic/DMEM | 1.662 |
| Tissue culture plastic/OS | 1.515 |
| Collagen I/DMEM | 1.763 |
| Collagen I/OS | 2.372 |
| Vitronectin/DMEM | 2.767 |
| Vitronectin/OS | 2.761 |
