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. 2016 Jun 29;9(7):524. doi: 10.3390/ma9070524

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Response surface plots of total alkaloids yields showing: (a) the effect of time and temperature at constant pressure (20 MPa) and ethanol concentration (90%); (b) the effect of time and pressure at constant temperature (55 °C) and ethanol concentration (90%); (c) the effect of time and ethanol concentration at constant temperature (55 °C) and pressure (20 MPa); (d) the effect of temperature and pressure at constant time (2.5 h) and ethanol concentration (90%); (e) the effect of temperature and ethanol concentration at constant time (2.5 h) and pressure (20 MPa); and (f) the effect of pressure and ethanol concentration at constant time (2.5 h) and temperature (55 °C).