Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Feb;187(3):1196–1200. doi: 10.1128/JB.187.3.1196-1200.2005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, American Society for Microbiology

PMC Copyright notice

FIG. 1. — Southern blot (A) and electrophoretic (B) analysis of the M.BtaII-induced methylation of ΦE125. (A) Approximately 50 μg of the indicated genomic DNA and ∼10 μg of ΦE125 purified from plate lysates were subjected to digestion by HindIII (H) according to the NEB-specified conditions. (B) Subsequently, half of each reaction mixture was removed and digested with McrBC (HM) under NEB-specified conditions. The DNAs were blotted by using the alkaline transfer protocol of the TurboBlotter (Schleicher & Schuell, Piscataway, N.J.) and photoimmobilized. The ECL probe (ΦE125::HindIII) was synthesized, hybridized, and detected according to the instructions specified by Amersham. The positive controls for McrBC digestion were E27 genomic DNA (lanes 2) and the NEB-supplied positive control (lane 13). The empty arrows indicate the chromosomal junction fragments and the host chromosome, while the filled arrow indicates the fragment that originates from excised viral genomes.