TABLE 1.
Strains, plasmids, and oligonucleotides
| Strain, plasmid, or oligonucleotide | Description | Reference(s) and/or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| TOP10 | Cloning strain | Invitrogen |
| SM10 | Mobilizing, tra (Kmr) | 17 |
| DB24 | Lacks all genomic DNA methylation | 11 |
| B. thailandensis | Nonpathogenic soil saprophyte | |
| E27 | 3, 18, 23 | |
| E125 | ||
| B. mallei | Soleploid and human pathogen | |
| ATCC 23344 | Human disease isolate; Cms Kms | 24; ATCC |
| BML10 | ΦE125-resistant ATCC 23344 | 22 |
| Plasmids | ||
| pCR2.1 | Topoisomerase-mediated cloning, Apr Kmr | Invitrogen |
| pAM1 | pCR2.1 lacZ-gp27 methylase; Apr Kmr | 22 |
| pCM1 | pCR2.1 lacZ-gp56 methylase; Apr Kmr | 22 |
| pBHR1 | Broad-host-range, mobilizing and expression (tra oriT Cmr Kmr) | MoBiTec |
| pAM15 | pBHR1: pcat-gp27; Cms Kmr | This study |
| pAM17 | pBHR1: pcat-gp27; Cms Kmr | This study |
| pCM18 | pBHR1: pcat-gp56; Cms Kmr | This study |
| pCM38 | pBHR1: pcat-gp56; Cms Kmr | This study |
| pDW18 | pBluescript KS containing 12,983-bp HindIII fragment from ΦE125; Apr | 22 |
| Oligonucleotides | ||
| attP F1 | GCACGCTGAAGAGTGAGGATG | This study |
| attP R1 | CATACGAGCCAAGGCCCTAAC | This study |
| 16S 5′ | 3 | |
| 16S 3′ | 3 | |
| B.th_rDNA F1 | CCGGCCTCAACCTGGCAACTGC | This study |
| B.th_rDNA R1 | GAGCCGACATCGAGGTGCCAAACA | This study |