Figure 3.

Strontium effect on Gro-α production by human primary bone cells. (A) CXCL1 and mRNA production were evaluated by RT-PCR analysis performed (as described in the Materials and Methods section) after 7, 14, and 21 days of stimulation by BCP, BCP_5%, and SrCl₂. Experiments were performed on 5 independent donors in duplicate. Data are shown as specific variation of target genes mRNA using the 2^−ΔΔCt method (HPRT1 was used as internal control); (B) Gro-α concentration was measured in cell supernatants after 7, 14, and 21 days of stimulation by BCP, BCP_5%, and SrCl₂. * means p < 0.05 when compared with control and ^# p < 0.05 when compared with BCP. Red bars represent median values. Black points represent maximum and minimum values. Black bars represent first and ninth deciles and the limits of white rectangle represents first and third quartiles.