TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype or characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| S. meliloti | ||
| Rm1021 | Wild type | 11 |
| RmF726 | Rm1021 ΔΩ5079-5149::Tn5-233 | 26 |
| WSUb20611-II | Rm1021 ΔdctA | This study |
| E. coli S17-1 | pro hsdR recA [RP4-2(Tc::Mu) (Km::Tn7)] | 20 |
| Plasmids | ||
| pSM100 | pCPP33 (1.5-kb BamHI dctA) | 27 |
| pK19mob-dctA-100 | pK19mob (dctA from pSM100) | This study |
| pSM100-32 | pSM100 (dctA G340T) | This study |
| pSM100-41 | pSM100 (dctA A975G) | This study |
| pSM100-53 | pSM100 (dctA C979T) | This study |
| pSM100-93 | pSM100 (dctA C265T C1034T) | This study |
| pK 19mob-dctA-93 | pK19mob (dctA from pSM100-93) | This study |
| pSM100-93-265 | pSM100 (dctA C265T) | This study |
| pSM100-93-1034 | pSM100 (dctA C1034T) | This study |
| pSYDCTII | 0.5-kb flanking dctA fragments cloned into pJPA22 in the BamHI site | This study |
| pJPA22 | pJQ200KS (BglII Tn10:tet) | 7 |
| pK19mob | pUC19 derivative lacZα, mob; Kmr | 19 |
a
Kmr, kanamycin resistance.