TABLE 3.
Transport of dicarboxylates by S. meliloti dctA mutants
| Strain |
Vmax (nmol [min mg of protein]−1) and Km (μM) valuesa on medium plus:
|
|||||
|---|---|---|---|---|---|---|
| Succinate
|
Fumarate
|
Malate
|
||||
| Vmax | Km | Vmax | Km | Vmax | Km | |
| RmF726(pSM100) | 32 | 51 | ND | ND | ND | ND |
| WSUb20611-II(pSM100) | 61 | 66 | 45 | 79 | 36 | 62 |
| WSUb20611-II(pSM100-32) | 30 | 457 | 21 | 145 | 17 | 706 |
| WSUb20611-II(pSM100-41) | 0.86 | 24 | 0.85 | 103 | 0.56 | 65 |
| WSUb20611-II(pSM100-53) | 40 | 321 | 40 | 155 | 40 | 771 |
| WSUb20611-II(pSM100-93) | 56 | 95 | 19 | 58 | 16 | 45 |
| WSUb20611-II(pSM100-93-1034) | 82 | 112 | 35 | 70 | 31 | 72 |
| WSUb20611-II(pSM100-93-265) | 81 | 92 | 37 | 46 | 29 | 72 |
a
Mutants were grown on M9 mannitol medium without induction. Km values are the micromolar concentrations of the corresponding substrate. ND, not determined.