TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or description | Reference or source |
|---|---|---|
| B. fragilis | ||
| ADB77 | TM4000; ΔthyA Tpr | 3 |
| CJB100 | ADB77:ΔrokA1 | This work |
| CJB101 | ADB77:ΔrokA1ΔhexA1 | This work |
| CJB200 | ADB77:ΔhexA1 | This work |
| ATCC 25285 | Subclone of NCTC 9343 | 26 |
| TAL2480 | Cefr | 12 |
| TAL3636 | Cefr | 12 |
| ATCC 23745 | rokA+hexA+ | 26 |
| TAL21616-21633 | Clinical isolates acquired from Tufts Anaerobic Laboratory | This work |
| B. thetaiotaomicron | ||
| VPI 8582 | rokA+hexA+ | 45 |
| E. coli | ||
| DH5α | λ nonlysogen | 44 |
| HB101 | rpsL20 | 38 |
| Plasmid | ||
| RK231 | RP4 derivative, Tetr, Tra+ | 17 |
| pYT102 | p15A ori, Cmr, RP4 oriT, B. fragilis suicide vector containing B. fragilis thyA+ Tetr | 37 |
| pJST61 | E. coli-B. fragilis shuttle vector, Ampr, Clnr | 38 |
| pKDel12 | pYT102 ΔrokA1 | This work |
| pHDe12 | pYT102 ΔhexA1 | This work |
| pBFROK10 | pJST61 with 2.4-kb fragment from TM4000 containing the rokA gene | This work |
| pBFROK40 | pJST61 with 1-kb fragment from TM4000 containing the rokA gene under the λ PL promoter | This work |
| pBFHEX10 | pJST61 with 1.5-kbp fragment from TM4000 containing the hexA gene | This work |
| pBFROKHIS | pJST61 with 1.1-kb fragment from TM4000 containing the rokA gene with an oligohistidine- tagged C terminus | This work |