TABLE 3.
Purification of RokA protein
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Fold purifi- cation |
|---|---|---|---|---|---|
| Streptomycin sulfate- treated cell extract | 588 (441)a | 410.3 (307.7)b | 0.93 | 100 | 1 |
| DEAE | 28.7 (9.7)a | 334.9 (113.2)b | 34.5 | 38 | 37.2 |
| Hydroxyapatite | 3.7 (1.3)a | 62.4 (21.4)b | 48 | 19 | 51.6 |
| Mono-Q | 0.29 | 20 | 68.6 | 93 | 73.9 |
a
Numbers in parentheses indicate the amount of protein used for the next purification step.
b
Numbers in parentheses indicate the total activity used for the next purification step.