Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Km and Vmax values of highly purified RokA kinase activity with various substrates
| Substrate | Km (mM) | Vmax (μmol/min/mg) |
|---|---|---|
| Glucose | 0.58 | 69 |
| NAG | 0.85 | 37 |
| Mannose | 1.75 | 50 |
| 2-Deoxyglucose | 1.82 | 1.4 |
| Fructose | 44.5 | 2.9 |
| ATP | 0.23a | 69a |
| manNAcc | NDb | ND |
| NANAc | ND | ND |
| maltosec | ND | ND |
| galactosec | ND | ND |
Values of Km and Vmax for ATP were measured with 6.7 mM glucose.
ND, not detected.
Concentration of sugars tested to measure the kinase activities was >50 mM.
