FIG. 2.
Increased hemolysis and reduced CAMP factor activity in CsrR mutant strains. Blood agar plates were inoculated with wild-type GBS strains 515 and 2603, CsrR mutant strains 515csrRΔ and 2603csrRΔ, and repaired mutant strains 515csrRΔrep and 2603csrRΔrep (labeled 515rep and 2603rep in the figure, respectively). (A) Blood agar plate demonstrating increased zones of beta-hemolysis surrounding colonies of CsrR mutant strains 515csrRΔ and 2603csrRΔ. (B) CAMP test demonstrating reduced synergistic hemolysis at the intersection of streaks of CsrR mutant strains 515csrRΔ and 2603csrRΔ with a perpendicular streak of beta-lysin-producing S. aureus. The increased level of hemolysis by the mutant strains is also evident along the GBS streaks.