Fig 5.

The profibrotic phenotype of IPF fibroblasts can be normalized by neutralization of LXR. Synchronized normal (n = 4) and IPF (n = 6) primary lung fibroblasts were cultured with 1% FCS (%S). A, IPF fibroblasts contained higher concentrations of LXRα protein. B, Collagen production by IPF fibroblasts was (Fig 5, B) reduced by 22(S)HC or potentiated by GW3965 (C). D,LXRα siRNA reduced collagen production by IPF fibroblasts. E, 22(S)HC inhibited TGF-β production. F, GW3965 potentiated serum-induced ARG2 expression by IPF fibroblasts. G,Lxrα siRNA reduced ARG2 expression by IPF fibroblasts. Data are presented as mean ± SEM of 4 biological replicates. *P < .05.