FIG. 1.

Northern blot analysis of cid and lrg transcription in S. aureus UAMS-1. Total cellular RNA was isolated from UAMS-1 cells cultured in NZY broth in either the presence of 35 mM glucose or in the absence of glucose at 2, 3, 4, 6, 8, and 12 h postinoculum (as indicated above each lane of the blot). Ten micrograms of each RNA sample was separated through a 1% (wt/vol) agarose-formaldehyde gel, transferred to a nylon membrane, and hybridized to cidA-, cidB-, and lrgA-specific DIG-labeled probes. The sizes of each transcript were determined by comparison to an RNA ladder (Invitrogen) run on the same gel. It should be noted that the corresponding culture cell density (as measured by the OD₆₀₀), pH, and acetate concentration for each time point in this experiment are represented in Fig. 5.