FIG. 6.

Transcription of cidABC and lrgAB is increased by the presence of acetic acid in S. aureus UAMS-1. Total cellular RNA was isolated from UAMS-1 grown to late exponential growth phase (4 h postinoculum) in either NZY pH 7.5 (lane 1), NZY pH 7.5 plus 35 mM glucose (lane 2), NZY pH 5.0 (HCl) (lane 3), or NZY pH 5.0 (acetic acid) (lane 4), and 10 μg of each sample was separated through a 1% (wt/vol) agarose-formaldehyde gel, transferred to a nylon membrane, and hybridized to cidA- and lrgA-specific DIG-labeled probes. The sizes of each transcript were determined by comparison to an RNA ladder (Invitrogen) run on the same gel.