Fig. 3. Leukocyte density varies within extrahepatic transplant sites 4 weeks after hydrogel transplantation.
(A) Extrahepatic transplant site tissue explanted at week 4 after implantation was stained for CD45 (white) and CD11b (magenta) and was imaged for functional vasculature [lectin (green)] and cell nuclei [DAPI (4′,6-diamidino-2-phenylindole) (blue)]. Scale bars, 50 μm. (B) CD11b and CD45 staining was quantified and normalized to FOV area. n = 4 to 7 subjects per group. *P < 0.05, **P < 0.01, and ***P < 0.001, evaluated by Kruskal-Wallis nonparametric tests with Dunn’s multiple comparison.