FIG. 2.

(A) Growth of H. elongata wild-type (WT) and trk deletion mutants KB12 (ΔtrkI) and KB15 (ΔtrkH) on minimal medium at different salinities (0.51, 1.03, and 2.05 M NaCl) and low (0.1 mM) and high (5 mM) K⁺ concentrations. Growth was scored after 3 days (0.51 and 1.03 M NaCl) and 4 days (2.05 M NaCl) of incubation at 30°C. Mutant KB15 grew similarly to the wild type in low and high K⁺ at all salinities, while growth of KB12 accumulating K⁺ only via TrkH was hampered in low-K⁺ medium, especially at high salinity. (B) Growth of H. elongata wild type, trkA deletion mutant KB14, and KB12.2 (ΔtrkH ΔtrkI) on minimal medium at 1.03 M NaCl and different K⁺ concentrations (0.25 and 200 mM). For (B), growth was scored after 3 days' incubation at 30°C.