TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or descriptiona | Source or reference |
|---|---|---|
| H. elongata | ||
| DSM 2581T | Type strain | DSMZb |
| KB12 | ΔtrkI | This study |
| KB12.2 | ΔtrkI ΔtrkH | This study |
| KB14 | ΔtrkA | This study |
| KB15 | ΔtrkH | This study |
| KB16 | ΔtrkAH ΔtrkI | This study |
| E. coli | ||
| TK2420 | trkA trkD1 Δ(kdpFAB)5 trkG82 trkH1 | W. Epstein, University of Chicago |
| TK2691 | trkH trkG trkD1 Δ(kdpFAB) Δ(trkA) | W. Epstein, University of Chicago |
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF) U169 recA1 hsdR17 (rK− mK+) supE44 Δthi-1 gyrA relA1 | 15 |
| S17-1 | thi pro hsdR hsdM+ recA; Tpr Smr | 36 |
| Plasmids | ||
| pHSG575 | lacZ′, Cmr | 39 |
| pKH4 | pHSG575::8.5-kb chromosomal Sau3A-hydrolyzed DNA from H. elongata carrying trkI, Cmr | This study |
| pKA2-30 | pHSG575::8.5-kb chromosomal Sau3A-hydrolyzed DNA from H. elongata carrying trkAH, Cmr | This study |
| pK18mobsacB | Kmrmob sacB | 33 |
| pAKB7 | 2,172-bp PCR-fragment in pK18mobsacB for trkI deletion containing 1,071-bp upstream and 1,101 bp downstream of trkI; Kmr | This study |
| pAKB8 | 2,229-bp PCR-fragment in pK18mobsacB for trkA deletion containing 1,108 bp upstream and 1,121 bp downstream of trkA; Kmr | This study |
| pAKB9 | 2,323-bp PCR-fragment in pK18mobsacB for trkH deletion containing 1,162 bp upstream and 1,161 bp downstream of trkH; Kmr | This study |
| pAKB10 | 2,355-bp PCR fragment in pK18mobsacB for trkH deletion in ΔtrkA strain KB14, contains 1,194 bp upstream and 1,161 bp downstream of trkH; Kmr | This study |
a
Abbreviations of antibiotics: Cm, chloramphenicol; Km, kanamycin; Sm, streptomycin.
b
DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.