Figure 2.
In vivo antitumor assays conducted by the in-house developed temperature tuner.
Notes: (A) The setting up of tumor model; (B) the comparison of antitumor assays between low and high temperature; and (C) the illustration of how to tune the tumor temperature. The left panel in (B) shows the experimental heating device for enhancing in vivo tumor temperature. This device was composed of 1 thermostatic water bath box and 5 test tubes (50 mL distilled water). When tumors had reached about 150 mm3, mice were administered with intravenous injection of STN. After 1 hour, the tumors on the mice of STN (40°C) group were immersed in the test tubes (50 mL distilled water in 40°C) for 10 minutes. The heating process was performed three times (10-minute interval) by five manipulators dependently.
Abbreviations: STN, shikonin-loaded thermosensitive nanomicelle; VPTT, volume phase transition temperature.