Fig 3. Involvement of pregnane X receptor (PXR) in the effect of miR-148a on total bile acid (TBA) secretion induced by estradiol.
PXR mRNA expression was detected by quantitative polymerase chain reaction. (B)PXR the protein expression was detected by western blotting. (C) Rifampin (10 μmol/L) reversed the effect of estradiol on TBA secretion in the medium. Values represent the mean ± standard error of the mean (n = 3). +P < 0.05, ++P < 0.01 vs. control group, *P < 0.05, **P < 0.01 vs. estradiol (500 nmol/L, 12 or 48 h).