Figure 6. Increased H3K27me3 deposition occurs specifically in late-stage TE cells.

A) Tbx21, Tcf7, Foxo1, Bach2, and Id3 mRNA were measured in purified naïve (day 0) Ezh2^f/f (solid) P14⁺ CD8⁺ T cells, and in Ezh2^f/f (solid) and Ezh2^f/f GzmBCre⁺ (open) P14⁺ CD8⁺ T cells from day 4.5 p.i. using qRT-PCR. Data are representative of 3 independent experiments (n=2–3 mice/group/experiment).

B) WT P14⁺ CD8⁺ T cells were purified from naïve (day 0) and LCMV-Armstrong infected mice at days 1.5, 4.5 (KLRG1^Hi and KLRG1^Lo populations), 10 (KLRG1^HiIL7R^Lo TE and KLRG1^LoIL7R^Hi MP populations), and 30 p.i., and then ChIP-qPCR was performed for H3K27me3 using primers to the Tcf7 TSS and Bach2 intron 1 (black). Region within Ttn served as a negative control (white) for H3K27me3 based on genome-wide H3K27me3 ChIP-seq analysis. Data are cumulative of four independent experiments (n=2–4 mice/group/experiment) with % of input for each sample normalized internally to the % of input for the day 30 sample, thereby calculating fold enrichment relative to the d30 sample as plotted. Data are expressed as mean ± SD. *p<0.05, **p<0.01. See also Figure S6.