Use of the RExBAD approach to study the toxic gene ccdB. Strains TG1 (region 1), TG1 F′(ΔccdARExBADccdB) (region 2), and TG1 F′(ΔccdARExBADccdB)/pKKccdA (region 3) were streaked on LB medium, LB medium containing 0.4% glucose, and LB medium containing 0.4% arabinose, and the plates were incubated at 37°C for 16 h.