TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| TG1 | F′[traD36 proAB+lacIqlacZΔM15] supE hsdΔ5 thi Δ(lac-proAB) | Laboratory collection |
| TG1cat-araC | cat gene inserted downstream of the araC gene, Cmr | This study |
| TG1spec-araC | aadA7 gene inserted downstream of the araC gene, Specr | This study |
| TG1ΔtraA | F′ΔtraA::aphaIII, Kmr | Laboratory collection |
| TG1 F′(catRExBADtraY) | F′tra operon placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| TG1 F′(ΔccdAspecRExBADccdB) | F′ΔccdA, ccdB placed under control of the pBAD promoter of the specRExBAD cassette, Specr | This study |
| TG1catRExBADftsL | ftsL placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655 | l−rph-l | Laboratory collection |
| MG1655ΔlacIZ | ΔlacIZ::aphaIII, Kmr | Laboratory collection |
| MG1655ΔaraBADΔlacIZ | Ara− derivative of MG1655ΔlacIZ, Specr Kmr | This study |
| MG1655Δflu | Δflu::cat, Cmr | Laboratory collection |
| MG1655ΔoxyR | oxyR::aphaIII, Kmr | Laboratory collection |
| MG1655ΔoxyRΔflu | oxyR::aphaIII Δflu::cat, Kmr Cmr | Laboratory collection |
| MG1655catRExBADlacZ | lacZ placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655ΔaraBADcatRExBADlacZ | Ara− derivative of MG1655 catRExBADlacZ, Specr Kmr | This study |
| MG1655catRExBADflu | flu placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655ΔoxyRcatRExBADflu | P1vir transduction of ΔoxyR in MG1655 catRExBADflu, Kmr Cmr | This study |
| MG1655ΔypjA | ΔypjA::aphaIII, Kmr | This study |
| MG1655ΔyfaL | ΔyfaL::aphaIII, Kmr | This study |
| MG1655ΔycgH | ΔycgH::aphaIII, Kmr | This study |
| MG1655ΔydhQ | ΔydhQ::aphaIII, Kmr | This study |
| MG1655ΔycgV | ΔycgV::aphaIII, Kmr | This study |
| MG1655ΔydeKU | ΔydeKU::aphaIII, Kmr | This study |
| MG1655ΔyeeJ | ΔyeeJ::aphaIII, Kmr | This study |
| MG1655catRExBADypjA | ypjA placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655catRExBADyfaL | yfaL placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655catRExBADycgH | ycgH placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655catRExBADydhQ | ydhQ placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655catRExBADycgV | ycgV placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655catRExBADydeKU | ydeKU placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| MG1655catRExBADyeeJ | yeeJ placed under control of the pBAD promoter of the catRExBAD cassette, Cmr | This study |
| Plasmids | ||
| pKOBEG | pSC101 ts, araC arabinose-inducible λ redγβα operon, Cmr | 4 |
| pKOBEGA | Like pKOBEG but Ampr instead of Cmr | 4 |
| pKKccdA | ccdA cloned in the pKK223-3 plasmid, pTac controlled, Ampr | L. Van Melderen |