TABLE 1.
Bacterial strain and plasmid list
| Strain | Plasmid | Genotype or relevant characteristicsa | Reference or source |
|---|---|---|---|
| Porphyromonas gingivalis | |||
| ATCC 33277 | Wild type, type strain | ATCCb | |
| Δ695 | pg0695-deletion mutant from ATCC 33277, Cmr | This study | |
| Δ694 | pg0694-deletion mutant from ATCC 33277, Cmr | This study | |
| Δ695-694 | pg0695- and pg0694-deletion mutant from ATCC 33277, Cmr | This study | |
| abfD mutant | abfD mutant of ATCC 33277 with an ermF-ermAM insertion in abfD, Emr | This study | |
| W83 | Wild type, the genome sequenced | 31 | |
| Escherichia coli | |||
| TOP10 | As chemically competent cells, F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen Corporation | |
| DH5α | pKD260 | Derivative of pACYC184 with deletion of a 1.1-kbp HincII fragment, bearing cat gene, Cmr | Gift from K. Nakayamac |
| pCR-Blunt II-TOPO | Cloning vector, linearized with DNA topoisomerase I bound to the 3′ end of each DNA strand, Kmr | Invitrogen Corporation |
a
Cmr, chloramphenicol resistance; Emr, erythromycin resistance; Kmr, kanamycin resistance.
b
ATCC, American Type Culture Collection.
c
Division of Microbiology and Oral Infection, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences.