FIG. 1.

Schematic representation of the genetic organization of the sigV-rsiV chromosomal region of E. faecalis JH2-2. (A) Large arrows represent the ORFs, and their orientation shows the transcriptional direction. The nucleotide sequences of the sigV promoter region and of the putative Rho-independent terminators (T₁ and T₂) are shown. Nucleotides corresponding to the differences observed in the E. faecalis V583 genome sequence are in parentheses. Primers used for amplification of the overall DNA of the sigV locus (S7 and S8) and for mapping of the TSS (SP1 to SP3) are represented. The TSS (+1) and the putative −35 and −10 motifs are boxed. (B) Electropherogram obtained from a 5′ RACE PCR experiment. The sequence in the electropherogram was obtained with primer SP3 and dA-tailed cDNA. The last base (C) upstream of the dA tail corresponds to the first nucleotide transcribed (TSS).