Multiple alignment of ECF sigma factors SigV of E. faecalis (EfaSigV) and SigV (BsuSigV), SigW (BsuSigW), and SigX (BsuSigX) of B. subtilis. Highly conserved regions 2 and 4 are boxed, and the subdomains are indicated. Periods, colons, and asterisks indicate weakly similar, strongly similar, and identical amino acids, respectively.