TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| E. faecalis | ||
| JH2-2 | Fusr Rifr; plasmid-free wild-type strain | 57 |
| S26 | JH2-2 isogenic derivative sigV mutant | This work |
| AS39 | JH2-2 isogenic derivative rsiV mutant; 75-bp rsiV median deletion | This work |
| SAS | JH2-2 isogenic derivative sigV-rsiV double mutant; 743-bp median deletion within sigV-rsiV operon | This work |
| V583 | Clinical isolate; Vanr | 43 |
| E. coli | ||
| EC101 | KanrsupE thi (lacproAB) (F′ traD36 proAB lacIqZΔM15) repA | 28 |
| XL-1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| Plasmids | ||
| pORI19.1 | pWV01 derivative; Emr Ori+ RepA−lacZ′ | 28 |
| pG+host3 | pWV01 derivative; Cmr repAts (previously named pVE6007) | 31 |
| pMSP3535 | EmrnisR nisK PnisA (nisin-inducible promoter) | 4 |