. 2005 Feb;187(3):923–929. doi: 10.1128/JB.187.3.923-929.2005

TABLE 1.

Strains, media, and sources

Type species	Medium	Source^a
Haloarcula vallismortis	ATCC 1863^b^,^c	ATCC 29715
Halobacterium salinarum (NRC-1)^d	GN101(8)	S. DasSarma
Halobaculum gomorrense	ATCC 2169^b	ATCC 700876
Halococcus morrhuae	ATCC 1863^b^,^c	ATCC 43103
Haloferax volcanii (DS70)^d	ATCC 1176^b	C. Daniels
Halogeometricum borinquense	ATCC 213^b^,^e	R. Vreeland
Halorubrum saccharovorum	ATCC 876^b^,^f	R. Vreeland
Haloterrigena turkmenica	ATCC 1863^b^,^c	R. Vreeland
Natrialba asiatica	ATCC 805^b^,^g	C. Litchfield
Natrinema pellirubrum	ATCC 1863^b^,^c	R. Vreeland
Natronobacterium gregoryi	ATCC 1590^h	P. Jablonski
Natronococcus occultus	ATCC 1590^h	P. Jablonski
Natronomonas pharaonis	ATCC 1590^h	P. Jablonski
Natronorubrum bangense	ATCC 1590^h	JCM 10635

ATCC, American Type Culture Collection; JCM, Japan Collection of Microorganisms.

These media were prepared by using ATCC recipes (http://www.atcc.org) with the following modifications: media were filter sterilized, and trace elements (0.2 μM CuSO₄ · 5H₂O, 11.6 μM Fe(NH₄)₂(SO₄)₂ · 6H₂O, 1.8 μM MnSO₄ · H₂O, 1.5 μM ZnSO₄ · 7H₂O) were added. Trace elements were aliquoted, stored at −20°C, and added just prior to inoculation. Trace elements were thawed only once.

FeCl₂ and MnCl₂ were excluded.

NRC-1 was chosen because its genome has been sequenced (18); DS70 was chosen because it is widely used to study haloarchaeal genetics.

Made as one solution. CaCl₂ · 2H₂O was used in place of CaCl₂ · 6H₂O (filter sterilized using a prefilter).

Autoclaved, cooled to room temperature, and then filter sterilized using a prefilter.

FeSO₄ was excluded.

These media were prepared by using ATCC recipes with the following modifications: media were filter sterilized and contained SL-6 trace elements (3.5 μM ZnSO₄ · 7H₂O, 1.5 μM MnCl₂ · 4H₂O, 48.5 μM H₃BO₃, 8.4 μM CoCl₂ · 6H₂O, 0.59 μM CuCl₂ · 2H₂O, 0.84 μM NiCl₂ · 6H₂O, 1.3 μM Na₂MoO₄ · H₂O). Trace elements were aliquoted, stored at −20°C, and added just prior to inoculation. Trace elements were thawed only once. The medium was incubated at 41°C overnight, cooled to room temperature, and then filter sterilized using a prefilter.