Figure 1.

miR-375 level is downregulated and negatively correlated with JAK2 expression in DRG after chronic morphine treatment. (A and B) qRT-PCR is used to detect the levels of miR-375 in DRG (A) and spinal cord (B). (C and D) JAK2 mRNA and protein levels are examined by qRT-PCR and Western blot assays in DRG after chronic morphine treatment. (E and F) qRT-PCR and Western blot analysis are used to test the mRNA (E) and protein (F) levels of JAK2 in spinal cord after chronic morphine treatment. (G) qRT-PCR results show that miR-375 level is negatively correlated with JAK2 mRNA level in DRG following morphine treatment. (H and I) miR-375 level is detected in DRG (H) and spinal cord (I) after acute morphine treatment. (J–L) JAK2 mRNA and protein expression levels are examined in DRG (J and L upper) and spinal cord (K and L lower) following acute morphine treatment. Data were presented as mean ± SD; **P<0.01 vs. control.

Abbreviations: DRG, dorsal root ganglia; mRNA, messenger RNA; JAK2, Janus kinase 2; qRT-PCR, quantitative real-time polymerase chain reaction; SD, standard deviation.