Figure 5. ⁸⁹Zr-labeled GFP⁺ pre-transplanted BM cells are mobilized into the blood by plerixafor/G-CSF treatment.

Two groups of mice were pre-transplanted with ⁸⁹Zr-labeled GFP⁺ BM cells (2×10⁷ cells at 37–111 kBq/10⁶ cells). One group of mice received i.v. injections of plerixafor/G-CSF at 3 h and 1 d and blood was collected 2h after the second mobilization treatment from both experimental and control groups. A. ⁸⁹Zr activity associated with cells in the blood of experimental group (black) increased by 3.8-fold compared to control (white) (n=4; P = 0.0009). B. Flow cytometry analysis confirmed that BM cell mobilization increased GFP⁺ cell fraction in the circulation (n=4, white: control, black: mobilized).