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. 2017 Jun 2;7:112. doi: 10.1186/s13568-017-0410-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — The expression analysis of anti-CD22 scFv–apoptin in the soluble and insoluble fractions of lysed bacterial cells. a SDS-PAGE analysis: protein molecular weight standards (lane 1), total protein from E. coli BL2 (DE3) containing pET-28 a (+) vector (negative control) grown under identical condition (lane 2), insoluble fraction (lane 3), and supernatant of cell lysate (lane 4) of induced E. coli BL2 (DE3) containing pET-anti-CD22 scFv–apoptin. A major band of ~44 kDa in insoluble fraction indicates anti-CD22 scFv–apoptin (white arrow). b Western blot analysis: the samples were loaded as in a