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. 2017 Jun 2;7:2687. doi: 10.1038/s41598-017-02461-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Zebrafish coronary vessels continue to grow after 3 months post fertilization. (a,b) Representative brightfield images of adult zebrafish and maximum intensity projections of confocal images of freshly isolated hearts from 110 (a) and 230 (b) dpf Tg(fli1a:EGFP) animals. Green cells have pan-endothelial identity. (c) Quantification of cardiac ventricular surface area covered by endothelial cells (n = 3 of each stage, mean ± SEM). One way ANOVA followed by Bonferroni’s post-hoc test (GraphPad Prism) was performed to evaluate statistical significance of differences. P < 0.05 was considered statistically significant. ** corresponds to P < 0.05. AVC- atrioventricular canal, dpf- days post fertilization.