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. 2017 Jun 2;7:2687. doi: 10.1038/s41598-017-02461-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scratch/wound-healing assay on cardiac endothelial cells. Assay was performed on isolated cardiac endothelial cells from adult Tg(kdrl:EGFP) zebrafish ventricles. (a) Fluorescence and brightfield images at 4 and 16 h after scratching. (b) Quantification of the initial cell free area covered by endothelial cell migration in 12 h. Cell free area at 4 h after scratch was considered as 100% (n = 3, mean ± SEM). One way ANOVA followed by Bonferroni’s post-hoc test (GraphPad Prism) was performed to evaluate statistical significance of differences. P < 0.05 was considered statistically significant. *** corresponds to P < 0.001.