Table 1.
MS parameters for SRM detection of HT4 and LT3 peptides as well as corresponding internal standards.
| Peptide Sequence | Average mass (Da) | Precursor ion m/z | Product ion m/z | Collision energy (eV) | Retention time (min) |
|---|---|---|---|---|---|
| GLEWLGVIWSGGNTDYNTPFTSR | 2570.8 | 857.6 (z = 3) | 908.6 (y16 +2 ) | 16 | 8.8 |
| 851.6 (y15+2) | 16 | 8.8 | |||
| 759.0 (y14+2) | 16 | 8.8 | |||
| GLEWLGVIWSGGNTDYNTPFTSR[13C6;15N4] | 2580.8 | 860.9 (z = 3) | 913.6 (y16 +2 ) | 16 | 8.8 |
| 857.2 (y15+2) | 16 | 8.8 | |||
| 764.0 (y14+2) | 16 | 8.8 | |||
| ASQSIGTNIHWYQQR | 1788.9 | 597.2 (z = 3) | 652.0 (y10 +2 ) | 15 | 3.6 |
| 708.6 (y11+2) | 15 | 3.6 | |||
| 752.2 (y12+2) | 15 | 3.6 | |||
| ASQSIGTNIHWYQQR[13C6;15N4] | 1798.9 | 600.6 (z = 3) | 657.0 (y10 +2 ) | 15 | 3.6 |
| 713.6 (y11+2) | 15 | 3.6 | |||
| 757.2 (y12+2) | 15 | 3.6 |
In bold: Product ion selected for quantification. Additional product ions are used for verification.