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. 2017 Jun 2;7:2698. doi: 10.1038/s41598-017-02970-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Bar plot showing the distribution of the number of transcript isoforms and predicted ORFs per gene. (B) Heatmap representing the levels of isoform transcripts of 25 differentially alternatively spliced (DAS) genes. Digits indicate the number of spectra from isoform-specific peptides. (C) Heatmap reports the correlation between the level of transcription of alternative isoforms and the normalized values of spectra from isoform-specific peptides for gametophores (Gametophores_FPKM-G_peptides), protonemata (Protonemata_FPKM-N_peptides) and protoplasts (Protoplasts_FPKM-P_peptides); digits indicate Spearman correlation coefficients.