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. 2017 Jun 2;7:2698. doi: 10.1038/s41598-017-02970-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Venn diagram showing lncRNAs distribution in gametophores, protonemata and protoplasts. (B) Bar plot showing the length of interaction sites between lncRNA-mRNAs and lncRNA-pre-mRNAs. (C) Heatmap representing expression patterns of the potentially interacted lncRNAs and mRNAs in gametophores, protonemata and protoplasts. N_FPKM, G_FPKM, P_FPKM ≥indicating the expression profiles of mRNAs in protonemata, gametophores and protoplasts, respectively. Lnc.N, lnc.G and lnc.P indicating the expression profiles of lncRNAs in protonemata, gametophores and protoplasts, respectively; (D) Venn diagram representing the overlap of unique interaction sites between reference (Ref) and alternative (AS) isoforms. (E) Venn diagram showing the landscape of lncRNA:mRNA interaction sites in gametophores, protonemata and protoplasts. (F) Results of quantitative polymerase chain reaction (qRT-PCR) data for serine/arginine-rich (SR) genes after treatment of protonemata with 50 µM abscisic acid (ABA) and 400 µM methyl jasmonate (MeJA). Protonemata treated with ultrapure Milli-Q water were used as a control.