Table 2.
Frequency of substitution types produced during 20 PCR cycles.
| Polymerase | A > C, T > G | A > G, T > C | A > T, T > A | C > A, G > T | C > G, G > C | C > T, G > A |
|---|---|---|---|---|---|---|
| Encyclo | 4% | 59% | 5% | 2% | 1% | 29% |
| Kapa HF | 2% | 10% | 2% | 14% | 1% | 71% |
| SD-HS | 3% | 31% | 14% | 5% | 17% | 30% |
| SNP-detect | 5% | 27% | 1% | 2% | 1% | 64% |
| Taq-HS | 3% | 56% | 7% | 7% | 2% | 25% |
| Tersus-buffer1 | 5% | 35% | 2% | 6% | 1% | 52% |
| Tersus-buffer2 | 5% | 45% | 2% | 2% | 1% | 46% |
| TruSeq | 6% | 13% | 3% | 25% | 3% | 50% |
| KTN | 4% | 60% | 4% | 2% | 1% | 29% |
Relative frequency of each of six substitution types that can be inferred without strand information across all errors produced by each polymerase. We used pooled data from two independent experiments to construct the table, with frequencies normalized to the ratio of the corresponding base in the template sequence. The most frequent substitution for each polymerase is shown in bold.