Table 3.
Frequency of substitution types produced during linear amplification.
| Polymerase | A > C | A > G | A > T | C > A | C > G | C > T | G > A | G > C | G > T | T > A | T > C | T > G |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Encyclo | 6% | 22% | 3% | 10% | 2% | 19% | 25% | 1% | 1% | 1% | 11% | 1% |
| Kapa HF | 3% | 4% | 1% | 42% | 1% | 23% | 16% | 1% | 7% | 0% | 2% | 0% |
| SD-HS | 5% | 11% | 22% | 8% | 7% | 13% | 16% | 3% | 4% | 4% | 6% | 0% |
| SNP-detect | 8% | 9% | 0% | 5% | 2% | 26% | 42% | 0% | 1% | 1% | 6% | 0% |
| Taq-HS | 4% | 15% | 8% | 24% | 2% | 13% | 19% | 1% | 2% | 4% | 8% | 0% |
| Tersus-buffer1 | 9% | 6% | 0% | 22% | 1% | 18% | 38% | 1% | 1% | 1% | 4% | 0% |
| Tersus-buffer2 | 10% | 10% | 0% | 6% | 2% | 22% | 40% | 1% | 1% | 1% | 7% | 0% |
| TruSeq | 5% | 3% | 1% | 43% | 1% | 8% | 15% | 1% | 20% | 0% | 2% | 0% |
| KTN | 6% | 24% | 3% | 8% | 2% | 19% | 26% | 0% | 1% | 1% | 10% | 0% |
Relative frequency of each of twelve possible substitution errors produced by each polymerase. We used pooled data from two independent experiments to construct the table, with frequencies normalized to the ratio of the corresponding base in the template sequence. The most frequent substitution for each polymerase is shown in bold.