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. 2017 Jun 2;7:2701. doi: 10.1038/s41598-017-02953-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amino acid substitutions in the aminergic toxins and their ancestors from the two evolutionary pathways. Sequences are following the time/tree topology of the aminergic toxins. MT7 and MT2 sequences are not shown in the ρ-Da1a pathway due to divergences in sequence and function. Amino acid substitutions are highlighted in the first pathway. MT1-MT3 evolutionary pathway is also illustrated. Color display is settled in order to emphasize amino acid conservation along the toxins. Stronger blue color indicates residue conservation among all sequences.