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. 2017 Jun 2;7:2695. doi: 10.1038/s41598-017-02705-0

Figure 3.

Figure 3

Histological examination revealed that SA-β-catenin mice have better histological morphology at week 8 post-fracture. (A–D) Representative HE staining of the fracture site in WT, β-catenin-KO, CA-β-catenin and SA-β-catenin mice. (E–H) Representative Fast Green/Safranin O staining images of the fracture site in each group. (I–L) Representative sirius red staining images of the fracture site in each group. (M–P) Representative Masson staining images of the fracture site in each group. (Q–T) Representative Von Kossa staining images of the fracture site in each group. (U) The optical density of Masson staining and Von Kossa staining images of the fracture site in each group. (V) mRNA expression level of collagen I at the fracture site in each group. The values are expressed as the means ± SDs. *p < 0.05, **p < 0.01. (arrows in G: the cartilage matrix, Scale bar = 100 µm in A–H and M–P, 50 µm in I–L and Q–T, n = 6/group).