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. 2017 Jun 2;7:2733. doi: 10.1038/s41598-017-02864-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TPM imaging inside the 16.4T MRI magnet. (a) TPM images of microglia cells in a fixed CX3CR1 mouse brain (scale bar: 50 μm). (b–d) The cross-correlation peak value and peak positions of the time lapsed TPM images during which we performed MRI recording. The cross-correlation shows that the operation of MRI had no influence on TPM. (e) Cross-sectional plot of the process of microglia and its Gaussian fitting (blue line) to determine the FWHM values. (f) Ex vivo ultrahigh-resolution spin-echo multiple-slice MRI of the same fixed mouse brain (axial orientation, 117 μm × 117 μm in plane resolution, 1 mm slice thickness, and 2 signal averages) acquired at 16.4 T. (g) In vivo ultrahigh-resolution mouse brain image (102 μm × 102 μm in plane resolution, 0.5 mm slice thickness and 4 signal averages) acquired from a different mouse at 9.4T for comparison.