Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 2;7:2697. doi: 10.1038/s41598-017-03038-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ZIKV RdRp enzymatic activities using homopolymeric templates. (A) Template utilization by ZIKV RdRp. Radionucleotide incorporation was monitored using 3.5 pmoles of WT enzyme (fraction D7 from gel filtration, see Fig. 1B) and various template-primer combinations. (B) Effect of the metal cofactor on nucleotide incorporation. (C) Impact of increasing concentration of NaCl on radionucleotide incorporation. The assay was conducted in conditions similar to panel A using the poly(rC) substrate and increasing concentrations of MnCl₂ and NaCl, respectively. Because the RdRp was purified in a buffer containing NaCl, the lowest salt concentration reachable is 15 mM (no salt added). (D) De novo RNA synthesis activity of ZIKV RdRp throughout the purification stages. Pooled fractions eluted from the nickel column and from the heparin column were tested at 15 pmoles of total proteins for the WT and GND mutant polymerases. In parallel, fraction D7, corresponding to the main retention pic after gel filtration was assayed at 0.7, 1.4, 3.5 and 7 pmoles. Means and standard deviations derives from three independent determinations.