Figure 6.

Reduced Probability of Glutamate Release Is Rescued by Drd2 Antagonist at Thalamic Inputs to LA in 22q11DS Mice

(A) Image of an LA neuron filled with the Alexa Fluor 594 and Fluo-5F through the patch pipette. Scale bar, 50 μm.

(B) Image of a dendrite with dendritic spines in an LA neuron. The line shows the direction of a line scan. Scale bar, 2 μm.

(C) Representative fluorescence images of a line scan through an individual dendritic spine of an LA neuron. The green channel (G) shows Fluo-5F fluorescence. The red channel (R) shows Alexa Fluor 594 fluorescence. Scale bar, 50 ms.

(D and E) Examples of line scans as a function of time in the same dendritic spines of WT (D) and Df(16)1/+ (E) mice before and after application of the Drd2 antagonist L-741,626.

(F and G) Mean peak amplitudes (F) and probabilities (G) of calcium transients in dendritic spines, which are the sites of thalamic inputs to the LA in WT (nine spines, six animals) and Df(16)1/+ (ten spines, six animals) mice before and after the application of L-741,626. Two-way repeated-measures ANOVA: peak amplitude: F_1,17 = 0.031, p = 0.862. Probability: F_1,17 = 20.442, ^∗p < 0.001. Post hoc: WT, before versus after L-741,626, p = 0.754; Df(16)1/+, before versus after L-741,626, ^∗p < 0.001; WT (no L-741,626) versus Df(16)1/+ (no L-741,626), ^∗p < 0.001; WT (with L-741,626) versus Df(16)1/+ (with L-741,626), p = 0.201.