Figure 5.

Knockdown of RUNX1-EVI1 Results in Loss of the Expression of Stem Cell Genes and the Upregulation of Myeloid Genes

(A–H) RT-PCR analysis of mRNA levels of the indicated genes relative to GAPDH and normalized to untreated cells in SKH-1 cells after RUNX1-EVI1 siRNA as compared to control siRNA transfection. GATA2 (A), MEIS1 (B), HOXA9 (C), CEBPB (D), CSF1R (E), CTSG (F), MPO (G), and CEBPA (H). The graph shows mean and SEM of four independent experiments. n.s., not significant; ^∗p < 0.05; ^∗∗p < 0.01 (unpaired t test). See also Table S1.

(I) KEGG pathways highlighting genes and pathways that are upregulated after RUNX1-EVI1 knockdown.

(J) KEGG pathways highlighting pathways associated with genes with shared binding of GATA2, RUNX1, and RUNX1-EVI1 that are upregulated after RUNX1-EVI1 knockdown.

See also Figure S5.