Figure S1.

CM2 Assembles into a Helical Dimer in Solution that further Assembles into an Antiparallel Tetramer In Crystallo, Related to Figures 2 and 4

(A) An extended Multiple Sequence Alignment (MSA) of the CM2 domain: boxed residues are visible in the crystal structures. Residues that have been subjected to mutational analyses are highlighted with asterisks: color corresponds to the color illustrated in the crystal structures.

(B) Cartoon representation of the CM2 dimer, and space-filling diagram of the CM2 tetramer formed in crystallo. Several conserved residues are highlighted in color.

(C) SEC-MALS analysis of the purified CM2 domain at different concentrations, illustrating that the protein forms a stable dimer with little tendency to form higher-order oligomers even at high protein concentrations.