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. 2017 May 23;8:15403. doi: 10.1038/ncomms15403

Figure 2. Proliferation changes induced by KRAB-dCas9 are not prone to cleavage-induced toxicity.

Figure 2

(a) Distribution of proliferation changes induced by sgRNAs targeting the three top cage-seq TSS of 105 cell essential genes in HT29 cells expressing KRAB-dcas9. (b) Proliferation changes and locations of two alternative MYC TSSs (TSS_1 and TSS_2) in HT29 cells expressing KRAB-dCas9. Error bars represent the deviation from the mean of sgRNAs targeting the indicated gene. (c) Distribution of proliferation changes induced by sgRNAs targeting the AASV1 genomic region in HT29 cells expressing Cas9 (d) Distribution of proliferation changes induced by the same AAVS1-targeting sgRNAs from c in cells expressing KRAB-dCAs9. (e) Violin plot showing distribution of proliferation changes induced by sgRNAs that target multiple genomic regions in Cas9 (red) or KRAB-dCAs9 (blue) expressing cells. (f) Proliferation changes induced by Cas9 (blue) or KRAB-dCas9 (red) following suppression of genes within the 8q24 amplicon in HT29 cells. Gene score were calculated by the mean proliferation changes induced by all sgRNAs-targeting TSS_1 (KRAB-dCas9) or exon (Cas9). (g) Experiment as (f) but targeting the 17q12 amplicon. All FCs are presented as the mean of duplicate experiments.