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. 2017 May 23;8:15349. doi: 10.1038/ncomms15349

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(a) Comparison of the conserved HHxxxDG region in the C domains of four reported fungal iterative NRPSs. The active site H residues are highlighted. (b) HPLC analysis of the in vitro reaction of C_2(BbBEAS) with D-Hiv-SNAC (S1) and N-Me-L-Phe-SNAC (S2). (c) HPLC analysis of the in vitro reaction of C_1(BbBEAS) with S1 and S2. (d) HPLC analysis of the in vitro reaction of C_1(BbBEAS) with S1 and S4. (e) HPLC analysis of the products of C₁-mutated BbBEAS and BbBSLS in S. cerevisiae BJ5464-NpgA.